Product Name

CD68 antibody

Documents

Description

CD68 Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue, human liver cancer tissue. Positive FC detected in RAW 264.7 cells.

Tested applications

ELISA, IHC, FC

Species reactivity

Human, Mouse; other species not tested.

Alternative names

CD68 antibody; CD68 molecule antibody; DKFZp686M18236 antibody; GP110 antibody; Macrosialin antibody; SCARD1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of CD68 recombinant protein (Accession Number: NM_001251). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

IHC: 1:20-1:200

Validations

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109134(CD68 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109134(CD68 Antibody) at dilution of 1:50 (under 40x lens)

1X10^6 RAW 264.7 cells were stained with 0.2ug CD68 antibody (Catalog No:109134, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Background

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

References

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Gao F, Chambon P, Tellides G, Kong W, Zhang X, Li W. Disruption of TGF-β signaling in smooth muscle cell prevents flow-induced vascular remodeling. Biochemical and biophysical research communications. 454(1):245-50. 2014.
Isobe A, Sawada K, Kinose Y. Interleukin 6 receptor is an independent prognostic factor and a potential therapeutic target of ovarian cancer. PloS one. 10(2):e0118080. 2015.
Zhou L, Chen H, Wen Q, Zhang Y. Indoleamine 2,3-dioxygenase expression in human inflammatory bowel disease. European journal of gastroenterology & hepatology. 24(6):695-701. 2012.
Liu G, Fiala M, Mizwicki MT. Neuronal phagocytosis by inflammatory macrophages in ALS spinal cord: inhibition of inflammation by resolvin D1. American journal of neurodegenerative disease. 1(1):60-74. 2012.
Zhou M, Ma C, Liu W. Valsartan Promoting Atherosclerotic Plaque Stabilization by Upregulating Renalase: A Potential-Related Gene of Atherosclerosis. Journal of cardiovascular pharmacology and therapeutics. 20(5):509-19. 2015.
Wang CN, Duan GL, Liu YJ. Overproduction of nitric oxide by endothelial cells and macrophages contributes to mitochondrial oxidative stress in adrenocortical cells and adrenal insufficiency during endotoxemia. Free radical biology & medicine. 83:31-40. 2015.
Dyugovskaya L, Berger S, Polyakov A, Lavie L. The development of giant phagocytes in long-term neutrophil cultures. Journal of leukocyte biology. 96(4):511-21. 2014.

CD68 antibody

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