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Product Name
CD4 antibody
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Description
CD4 Rabbit Polyclonal antibody. Positive IP detected in mouse spleen tissue. Positive WB detected in human spleen tissue. Positive IHC detected in human tonsillitis tissue. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IP, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CD4 antibody; CD4 molecule antibody; CD4mut antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD4 recombinant protein (Accession Number: NM_001382707). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:50-1:500
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Validations
human spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:109035(CD4 antibody) at dilution of 1:300
IP result of anti-CD4 (Catalog No:109035 for IP and Detection) with mouse spleen tissue lysate.
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109035(CD4 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109035(CD4 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"