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CD320 antibody

Cat.#: 109027

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Product Information

  • Product Name
    CD320 antibody
  • Documents
  • Description
    CD320 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HL-60 cells, human liver tissue, mouse heart tissue, Raji cells. Positive FC detected in HL-60 cells. Positive IHC detected in human tonsillitis tissue. Observed molecular weight by Western-blot: 50-55 kDa
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    8D6 antibody; 8D6 antigen antibody; 8D6A antibody; CD320 antibody; CD320 antigen antibody; CD320 molecule antibody; FDC signaling molecule 8D6 antibody; FDC SM 8D6 antibody; TCblR antibody; Transcobalamin receptor antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CD320 recombinant protein (Accession Number: NM_016579). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109027(CD320 antibody) at dilution of 1:500

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109027(CD320 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:109027(CD320 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:109027(CD320 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:109027(CD320 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:109027(CD320 antibody) at dilution of 1:50 (under 40x lens)

    1X10^6 HL-60 cells were stained with 0.2ug CD320 antibody (Catalog No:109027, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 HL-60 cells were stained with 0.2ug CD320 antibody (Catalog No:109027, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29kD. The 58kd size by SDS-PAGE suggests extensive glycosylation of the membrane-anchored protein.
  • References
    • Sysel AM, Valli VE, Nagle RB, Bauer JA. Immunohistochemical quantification of the vitamin B12 transport protein (TCII), cell surface receptor (TCII-R) and Ki-67 in human tumor xenografts. Anticancer research. 33(10):4203-12. 2013.
    • Sysel AM, Valli VE, Bauer JA. Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues. Oncotarget. 6(4):2331-48. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"