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Product Name
CD276 antibody
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Description
CD276 Rabbit Polyclonal antibody. Positive FC detected in RAW 264.7 cells. Positive IHC detected in human medulloblastoma tissue, human testis tissue. Positive WB detected in PC-3 cells, HEK-293 cells. Observed molecular weight by Western-blot: 100kd
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
4Ig B7 H3 antibody; B7 H3 antibody; B7 homolog 3 antibody; B7H3 antibody; CD276 antibody; CD276 antigen antibody; CD276 molecule antibody; Costimulatory molecule antibody; UNQ309/PRO352 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD276 recombinant protein (Accession Number: BC062581). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:109017(CD276 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human medulloblastoma using Catalog No:109017(CD276 antibody) at dilution of 1:100 (under 40x lens)
1X10^6 RAW 264.7 cells were stained with 0.2ug CD276 antibody (Catalog No:109017, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
CD276 represents CD276 molecule
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"