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Product Name
CD22 antibody
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Description
CD22 Mouse Monoclonal antibody. Positive IHC detected in human tonsillitis tissue, human lymphoma tissue. Positive WB detected in Raji cells. Observed molecular weight by Western-blot: 135 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
B cell receptor CD22 antibody; BL CAM antibody; CD22 antibody; CD22 molecule antibody; FLJ22814 antibody; SIGLEC 2 antibody; SIGLEC2 antibody; T cell surface antigen Leu 14 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of CD22 recombinant protein (Accession Number: NM_001278417). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:100-1:800
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:107053(CD22 antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107053(CD22 Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107053(CD22 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.
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