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Product Name
CD20 antibody
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Description
CD20 Rabbit Polyclonal antibody. Positive WB detected in Raji cells. Positive IP detected in Raji cells. Positive FC detected in Raji cells. Positive IHC detected in human tonsillitis tissue, human lymphoma tissue. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IHC, FC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
B lymphOCyte antigen CD20 antibody; Bp35 antibody; CD20 antibody; LEU 16 antibody; MS4A1 antibody; MS4A2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD20 recombinant protein (Accession Number: NM_152867). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
1X10^6 Raji cells were stained with 0.2ug MS4A1,CD20 antibody (Catalog No:109005, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-Goat anti-Rabbit IgG with dilution 1:100.
Immunohistochemistry of paraffin-embedded human tonsillitis slide using Catalog No:109005(MS4A1,CD20 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human tonsillitis slide using Catalog No:109005(MS4A1,CD20 Antibody) at dilution of 1:50
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:109005(MS4A1,CD20 Antibody) at dilution of 1:1000
IP Result of anti-MS4A1,CD20 (IP:Catalog No:109005, 4ug; Detection:Catalog No:109005 1:600) with Raji cells lysate 1440ug.
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Background
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
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