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CD20 antibody

Cat.#: 107050

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Special Price 239.0 USD

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Product Information

  • Product Name
    CD20 antibody
  • Documents
  • Description
    CD20 Mouse Monoclonal antibody. Positive IHC detected in human tonsillitis tissue, human lymphoma tissue. Positive FC detected in Raji cells. Positive WB detected in Raji cells. Observed molecular weight by Western-blot: 35 kDa
  • Tested applications
    ELISA, WB, FC, IHC
  • Species reactivity
    Human,Mouse; other species not tested.
  • Alternative names
    B lymphOCyte antigen CD20 antibody; Bp35 antibody; CD20 antibody; LEU 16 antibody; MS4A1 antibody; MS4A1 antibody; CD20 antibody; MS4A2 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of CD20 recombinant protein (Accession Number: NM_152867). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:100-1:400

  • Validations

    Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:107050(MS4A1,CD20 antibody) at dilution of 1:1000

    Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:107050(MS4A1,CD20 antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107050(MS4A1,CD20 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107050(MS4A1,CD20 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107050(MS4A1,CD20 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107050(MS4A1,CD20 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    1X10^6 Raji cells were stained with 0.2ug MS4A1,CD20 antibody (Catalog No:107050, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-mouse IgG with dilution 1:200.

    1X10^6 Raji cells were stained with 0.2ug MS4A1,CD20 antibody (Catalog No:107050, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-mouse IgG with dilution 1:200.

  • Background
    CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"