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Product Name
CD147 antibody
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Description
CD147 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in human liver tissue. Positive IHC detected in human lung cancer tissue, human breast cancer tissue, human gliomas tissue, human liver cancer tissue. Observed molecular weight by Western-blot: 34 kDa+50 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
5F7 antibody; Basigin antibody; basigin (Ok blood group) antibody; BSG antibody; CD147 antibody; Collagenase stimulatory factor antibody; EMMPRIN antibody; HAb18G antibody; OK blood group antigen antibody; TCSF antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD147 recombinant protein (Accession Number: NM_001322243). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109055(CD147 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:109055(CD147 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-CD147 (IP:Catalog No:109055, 4ug; Detection:Catalog No:109055 1:300) with HEK-293 cells lysate 2800ug.
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Background
BSG, also named as 5F7, Basigin, EMMPRIN, TCSF and CD147, plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS).CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. It seems to be a receptor for oligomannosidic glycans. CD147 is a receptor of CypA, inducing matrix metalloproteinase expression and mediating the degradation of the extracellular matrix, plays an important role in tumorigenesis and invasion in oral cancer. It has been considered as an objective and effective marker to predict invasion and prognosis in various cancers.
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References
- Huang C, Sun Z, Sun Y. Association of increased ligand cyclophilin A and receptor CD147 with hypoxia, angiogenesis, metastasis and prognosis of tongue squamous cell carcinoma. Histopathology. 60(5):793-803. 2012.
- Huang CF, Zhang L, Ma SR. Clinical significance of Keap1 and Nrf2 in oral squamous cell carcinoma. PloS one. 8(12):e83479. 2013.
- Wang Y, Yuan L, Yang XM. A chimeric antibody targeting CD147 inhibits hepatocellular carcinoma cell motility via FAK-PI3K-Akt-Girdin signaling pathway. Clinical & experimental metastasis. 32(1):39-53. 2015.
- Bu LL, Deng WW, Huang CF, Liu B, Zhang WF, Sun ZJ. Inhibition of STAT3 reduces proliferation and invasion in salivary gland adenoid cystic carcinoma. American journal of cancer research. 5(5):1751-61. 2015.
- Zhai Y, Wu B, Li J, Yao XY, Zhu P, Chen ZN. CD147 promotes IKK/IκB/NF-κB pathway to resist TNF-induced apoptosis in rheumatoid arthritis synovial fibroblasts. Journal of molecular medicine (Berlin, Germany). 94(1):71-82. 2016.
- Yan J, Mao Y, Wang C, Wang Z. Association Study between an SNP in CD147 and Its Expression With Acute Coronary Syndrome in a Jiangsu Chinese Population. Medicine. 94(42):e1537. 2015.
- Liu JF, Mao L, Bu LL. C4.4A as a biomarker of head and neck squamous cell carcinoma and correlated with epithelial mesenchymal transition. American journal of cancer research. 5(12):3505-15. 2015.
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