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Anti-CD147/EMMPRIN antibody

Cat.#: 102945

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Product Information

  • Product Name
    Anti-CD147/EMMPRIN antibody
  • Documents
  • Description
    Rabbit monoclonal to CD147/EMMPRIN
  • Tested applications
    ELISA, IF, ICC/IF
  • Species reactivity
    Mouse Basigin / BSG / CD147
    No cross-reactivity in ELISA with Human Basigin / BSG / CD147
  • Alternative names
    M6 antibody; OK antibody; 5F7 antibody; TCSF antibody; CD147 antibody; EMMPRIN antibody; HT-7 antibody; CD147 antibody; EMMPRIN antibody; AI115436 antibody; AI325119 antibody; 5F7 antibody; AI115436 antibody; AI325119 antibody; basigin antibody; basigin antibody; Bsg antibody; BSG antibody; CD147 antibody; CD147 antibody; EMMPRIN antibody; EMMPRIN antibody; HT-7 antibody; M6 antibody; OK antibody; TCSF antibody
  • Immunogen
    Mouse CD147/EMMPRIN (His Tag) recombinant protein
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD147 (rM CD147; NP_001070652.1; Met 1-Arg 209).
  • Clonality
    Monoclonal
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    ELISA: 0.1-0.2 μg/mL

    This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CD147. The detection limit for Mouse CD147 is approximately 0.00245 ng/well.

    ICC/IF: 10-25 μg/mL

  • Validations

    CD147 / EMMPRIN / Basigin Antibody, Rabbit MAb, Immunofluorescence

    CD147 / EMMPRIN / Basigin Antibody, Rabbit MAb, Immunofluorescence

    Immunofluorescence staining of Mouse BSG in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse BSG monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.

  • Background
    CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.
  • References
    • Tang Y, et al. (2004) Tumor-stroma interaction: positive feedback regulation of extracellular matrix metalloproteinase inducer (EMMPRIN) expression and matrix metalloproteinase-dependent generation of soluble EMMPRIN. Mol Cancer Res. 2(2): 73-80.
    • Wilson MC, et al. (2005) Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 280(29): 27213-21.
    • Curtin KD, et al. (2005) Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture. J Cell Sci. 118(Pt 12): 2649-60.
    • Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44. Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44.
    • Seizer P, et al. (2009) EMMPRIN (CD147) is a novel receptor for platelet GPVI and mediates platelet rolling via GPVI-EMMPRIN interaction. Thromb Haemost. 101(4): 682-6.
    • Moonsom S, et al. (2010) A Competitive ELISA for Quantifying Serum CD147: Reduction of Soluble CD147 Levels in Cancer Patient Sera. Hybridoma (Larchmt). 29(1): 45-52.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"