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Product Name
CCM2 Polyclonal Antibody
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Description
Polyclonal antibody to CCM2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CCM2 antibody; C7orf22 antibody; OSM antibody; PP10187 antibody; CCM2 scaffolding protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CCM2 (NP_113631.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CCM2 Polyclonal Antibody
Western blot analysis of extracts of MCF7 cells, using CCM2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - CCM2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver using CCM2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CCM2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using CCM2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CCM2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using CCM2 antibody at dilution of 1:100 (40x lens).
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Background
Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions (By similarity). May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"