-
Product Name
CBX4 antibody
- Documents
-
Description
CBX4 Rabbit Polyclonal antibody. Positive WB detected in Various cell, HEK-293 cells, PC-3 cells. Positive IF detected in U2OS cell, HeLa cells. Observed molecular weight by Western-blot: ~75 kDa
-
Tested applications
ELISA, IF, WB
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
CBX4 antibody; Chromobox protein homolog 4 antibody; E3 SUMO protein ligase CBX4 antibody; hPC2 antibody; NBP16 antibody; PC2 antibody; Polycomb 2 homolog antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of CBX4 recombinant protein (Accession Number: BC014967). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: N/AIF: N/A
-
Validations
WB result of anti-CBX4 (Catalog No:108946) with various cell. (Courtesy of L. Pirone and R. Barrio, CIC bioGUNE).
IF result of anti-CBX4 (Catalog No:108946, 1:200) with U2OS cell, MeOH fixation. (Courtesy of L. Pirone and R. Barrio, CIC bioGUNE).
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:108946(CBX4 Antibody) at dilution of 1:600
Immunofluorescent analysis of HeLa cells using Catalog No:108946(CBX4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"