Product Name

CBR3 antibody

Documents

Description

CBR3 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in HeLa cells, Jurkat cells. Observed molecular weight by Western-blot: 31kd

Tested applications

ELISA, WB, IHC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

Carbonyl reductase [NADPH] 3 antibody; carbonyl reductase 3 antibody; CBR3 antibody; hCBR3 antibody; SDR21C2 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of CBR3 recombinant protein (Accession Number: NM_001236). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108940(CBR3 antibody) at dilution of 1:500

Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108940(CBR3 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108940(CBR3 antibody) at dilution of 1:50 (under 40x lens)

Background

CBR3(Carbonyl reductase [NADPH] 3), which Belongs to the short-chain dehydrogenases/reductases (SDR) family, has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). The gene encodes a 31 kDa protein that can catalyze the reduction of many endogenous and xenobiotic carbonyl compounds, including steroids and prostaglandins. The deduced 277-amino acid CBR3 protein is 84% similar to the CBR1 protein(PMID:9740676).

References

Carlson BA, Yoo MH, Tobe R. Thioredoxin reductase 1 protects against chemically induced hepatocarcinogenesis via control of cellular redox homeostasis. Carcinogenesis. 33(9):1806-13. 2012.

CBR3 antibody

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