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CBP,EIF4E antibody

Cat.#: 108938

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Special Price 239.0 USD

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Product Information

  • Product Name
    CBP,EIF4E antibody
  • Documents
  • Description
    CBP,EIF4E Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human gliomas tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Positive WB detected in HEK-293 cells. Observed molecular weight by Western-blot: 25 kDa, 50 kDa
  • Tested applications
    ELISA, WB, IHC, IF, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CBP antibody; eIF 4E antibody; eIF 4F 25 kDa subunit antibody; EIF4E antibody; EIF4E1 antibody; EIF4EL1 antibody; EIF4F antibody; mRNA cap binding protein antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CBP,EIF4E recombinant protein (Accession Number: NM_001968). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108938(EIF4E antibody) at dilution of 1:1000

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108938(EIF4E antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108938(EIF4E antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108938(EIF4E antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using EIF4E antibody Catalog No:108938 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using EIF4E antibody Catalog No:108938 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    1X10^6 HepG2 cells were stained with 0.2ug EIF4E antibody (Catalog No:108938, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with 0.2ug EIF4E antibody (Catalog No:108938, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    Eukaryotic translation initiation factor 4E(EIF4E) is the rate-limiting part of mRNA 7-methyl guanosine cap-binding component of the translation initiation complex eIF4F. The other subunits of EIF4F are a 50-kD polopeptied, terned EIF4A and a 220-kD polypeptied EIF4G. EIF4E mediates the binding to the mRNA cap then repress the translation. Due to the increasing expression of c-Myc in the oncogene, EIF4E's activity is enhance. This is a rabbit polyclonal antibody raised against the full-length of human EIF4E. This protein can form a dimer (~50 kDa).
  • References
    • Zhao L, Teng B, Wen L. mTOR inhibitor AZD8055 inhibits proliferation and induces apoptosis in laryngeal carcinoma. International journal of clinical and experimental medicine. 7(2):337-47. 2014.
    • Wu DM, Zhang P, Xu GC. Pemetrexed induces G1 phase arrest and apoptosis through inhibiting Akt activation in human non small lung cancer cell line A549. Asian Pacific journal of cancer prevention : APJCP. 16(4):1507-13. 2015.
    • Cui Y, Xie S, Luan J, Zhou X, Han J. Identification of the receptor tyrosine kinases (RTKs)-oriented functional targets of miR-206 by an antibody-based protein array. FEBS letters. 589(16):2131-5. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"