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Product Name
CAV3 Polyclonal Antibody
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Description
Polyclonal antibody to CAV3
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CAV3 antibody; LGMD1C antibody; LQT9 antibody; VIP-21 antibody; VIP21 antibody; caveolin-3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human CAV3 (NP_001225.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CAV3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CAV3 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
Immunohistochemistry - CAV3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using CAV3 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - CAV3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using CAV3 antibody at dilution of 1:200 (40x lens).
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Background
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity). Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"