Cart 2

Cathepsin K antibody

Cat.#: 108893

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Cathepsin K antibody
  • Documents
  • Description
    Cathepsin K Rabbit Polyclonal antibody. Positive IF detected in ROS1728 cells, HepG2 cells. Positive IHC detected in human breast cancer tissue, human lung cancer tissue, human osteosarcoma tissue. Positive IP detected in ROS1728 cells. Positive WB detected in ROS1728 cells, mouse uterus tissue. Observed molecular weight by Western-blot: 29-36 kDa
  • Tested applications
    ELISA, IHC, WB, IP, IF
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    Cathepsin K antibody; Cathepsin O antibody; Cathepsin O2 antibody; Cathepsin X antibody; CTS02 antibody; CTSK antibody; CTSO antibody; CTSO1 antibody; CTSO2 antibody; PKND antibody; PYCD antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Cathepsin K recombinant protein (Accession Number: NM_000396). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    ROS1728 cells were subjected to SDS PAGE followed by western blot with Catalog No:108893(CTSK Antibody) at dilution of 1:1000

    ROS1728 cells were subjected to SDS PAGE followed by western blot with Catalog No:108893(CTSK Antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108893(CTSK antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108893(CTSK antibody) at dilution of 1:100 (under 10x lens)

    IP Result of anti-CTSK (IP:Catalog No:108893, 3ug; Detection:Catalog No:108893 1:500) with ROS1728 cells lysate 3200ug.

    IP Result of anti-CTSK (IP:Catalog No:108893, 3ug; Detection:Catalog No:108893 1:500) with ROS1728 cells lysate 3200ug.

    Immunofluorescent analysis of ROS1728 cells using Catalog No:108893(CTSK Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of ROS1728 cells using Catalog No:108893(CTSK Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    CTSK(cathepsin K), also named as CTSO, CTSO2, is a recently identified lysosomal cysteine proteinase. CTSK is synthesized as a proenzyme of 38 kDa and subsequently enters acidic lysosomal compartments, in which the propeptide is cleaved and transformed into an active enzyme and it may influence adipocyte differentiation through modifying extracellular matrix components(PMID:16912123). It is revealed both the 46-kDa cathepsin-K precursor and the 30-kDa mature form in mouse bone extracts(PMID:9811821). The high CTSK protein levels are only detected in primary cultured fibroblasts derived from normal and neoplastic breast tissue, where the 37- and 25-kDa bands to be detected correspond to pro- and mature proteins through western blot(PMID:18765527). The full length protein has a signal peptides with 15 amino acids and a propeptide with 99 amino acids. CTSK may have cross reaction with the other members of cathepsin family and form a complex of 70 kDa with an unidentified subunit(PMID:1930136).
  • References
    • Kozawa E, Nishida Y, Cheng XW. Osteoarthritic change is delayed in a Ctsk-knockout mouse model of osteoarthritis. Arthritis and rheumatism. 64(2):454-64. 2012.
    • Zhang D, Huang C, Yang C. Antifibrotic effects of curcumin are associated with overexpression of cathepsins K and L in bleomycin treated mice and human fibroblasts. Respiratory research. 12:154. 2011.
    • MacLellan LM, Montgomery J, Sugiyama F. Co-opting endogenous immunoglobulin for the regulation of inflammation and osteoclastogenesis in humans and mice. Arthritis and rheumatism. 63(12):3897-907. 2011.
    • Hirabara S, Kojima T, Takahashi N, Hanabayashi M, Ishiguro N. Hyaluronan inhibits TLR-4 dependent cathepsin K and matrix metalloproteinase 1 expression in human fibroblasts. Biochemical and biophysical research communications. 430(2):519-22. 2013.
    • Ho KY, Huang HH, Hung KF. Cholesteatoma growth and proliferation: relevance with serpin B3. The Laryngoscope. 122(12):2818-23. 2012.
    • Sumida K, Ubara Y, Hoshino J. Bone histomorphometry in a long-term hemodialysis patient with hypoparathyroidism and sarcoidosis. Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA. 26(4):1435-41. 2015.
    • Xue Y, Wang L, Xia D. Dental Abnormalities Caused by Novel Compound Heterozygous CTSK Mutations. Journal of dental research. 94(5):674-81. 2015.
    • Kozawa E, Cheng XW, Urakawa H. Increased expression and activation of cathepsin K in human osteoarthritic cartilage and synovial tissues. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 34(1):127-34. 2016.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"