Product Name

Catalase antibody

Documents

Description

Catalase Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells, HT-1080 cells, Jurkat cells, mouse liver tissue, Raji cells, rat liver tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human hepat℃irrhosis tissue. Observed molecular weight by Western-blot: 55-60kd

Tested applications

ELISA, IHC, WB, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

CAT antibody; catalase antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Catalase recombinant protein (Accession Number: NM_001752). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108884(CAT antibody) at dilution of 1:1000

Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:108884(CAT antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:108884(CAT antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of HepG2 cells using Catalog No:108884(Catalase Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Background

CAT(Catalase) belongs to the catalase family. It is involved in catabolic pathway of activated oxygen species and free radical detoxification and the catalase overexpression has a protective role against UVB irradiation by preventing DNA damage mediated by the late ROS increase. Mammalian catalase of approximately 240 kD occurs as a complex of 4 identical subunits, each of which contains 526 amino acid residues(PMID:19122680). Defects in CAT are the cause of acatalasemia (ACATLAS). This antibody is specific to CAT.

References

Wang C, Yue X, Lu X, Liu B. The role of catalase in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix. Fish & shellfish immunology. 34(1):91-9. 2013.
Wu H, Xiao Y, Zhang S. The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense. Cell reports. 3(5):1663-77. 2013.
Zhao D, Li Q, Huang Q. Cardioprotective effect of propofol against oxygen glucose deprivation and reperfusion injury in H9c2 cells. Oxidative medicine and cellular longevity. 2015:184938. 2015.
Yin Q, Zhu L, Liu D, Irwin DM, Zhang S, Pan YH. Molecular Evolution of the Nuclear Factor (Erythroid-Derived 2)-Like 2 Gene Nrf2 in Old World Fruit Bats (Chiroptera: Pteropodidae). PloS one. 11(1):e0146274. 2016.

Catalase antibody

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