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Product Name
CASC5 Polyclonal Antibody
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Description
Polyclonal antibody to CASC5
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Rat
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Alternative names
KNL1 antibody; AF15Q14 antibody; CASC5 antibody; CT29 antibody; D40 antibody; MCPH4 antibody; PPP1R55 antibody; Spc7 antibody; hKNL-1 antibody; hSpc105 antibody; kinetochore scaffold 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CASC5 (NP_733468.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - CASC5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CASC5 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - CASC5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat testis using CASC5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CASC5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using CASC5 antibody at dilution of 1:100 (40x lens).
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Background
Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"