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Product Name
CARM1 antibody
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Description
CARM1 Rabbit Polyclonal antibody. Positive IP detected in NIH/3T3 cells. Positive WB detected in COS-7 cells, HeLa cells, MCF7 cells, NIH/3T3 cells, PC-3 cells. Positive IF detected in NIH/3T3 cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 63-66 kDa,55 kDa
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Tested applications
ELISA, IHC, WB, IF, IP
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Species reactivity
Human,Mouse,Rat, Monkey; other species not tested.
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Alternative names
CARM1 antibody; PRMT4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_199141). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
COS-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:108861(CARM1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108861(CARM1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:108861(CARM1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-CARM1 (IP:Catalog No:108861, 4ug; Detection:Catalog No:108861 1:500) with NIH/3T3 cells lysate 2000ug.
Immunofluorescent analysis of NIH/3T3 cells using Catalog No:108861(CARM1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), β-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated through phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTMsites in cells themselves.
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