Canine PD-L1/B7-H1/CD274 (Fc Tag) recombinant protein

Canine PD-L1/B7-H1/CD274 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

PD-L1; CD274 A530045L16RIK; MGC142294; CD274; B7H1; PDCD1L1; MGC142296; PDL1; PDCD1LG1; B7-H

Human Cells

A DNA sequence encoding the canine CD274 (XP_541302.4) (Met1-Arg236) was expressed with the Fc region of human IgG1 at the C-terminus.

Canine

The recombinant canine CD274 consists 456 amino acids and predicts a molecular mass of 51.5 kDa.

> (17.0+77.6) % as determined by SDS-PAGE.

1.Measured by its binding ability in a functional ELISA.

2.Immobilized human PD1-His at 10μg/mL (100μL/well) can bind canine CD274-Fc, the EC50 of canine PD-L1-Fc is 0.1-0.5μg/mL.