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Product Name
CAMSAP1L1 antibody
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Description
CAMSAP1L1 Rabbit Polyclonal antibody. Positive IP detected in PC-3 cells. Positive WB detected in mouse brain tissue, HeLa cells, PC-3 cells. Positive IHC detected in human kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 168 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CAMSAP1L1 antibody; CAMSAP2 antibody; KIAA1078 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CAMSAP1L1 recombinant protein (Accession Number: BC065508). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108928(CAMSAP1L1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:108928(CAMSAP1L1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:108928(CAMSAP1L1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-CAMSAP1L1 (IP:Catalog No:108928, 4ug; Detection:Catalog No:108928 1:3000) with PC-3 cells lysate 5000ug.
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Background
CAMSAP1L1 represents calmodulin regulated spectrin-associated protein 1-like 1
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References
- Jiang K, Hua S, Mohan R. Microtubule minus-end stabilization by polymerization-driven CAMSAP deposition. Developmental cell. 28(3):295-309. 2014.
- Yau KW, van Beuningen SF, Cunha-Ferreira I. Microtubule minus-end binding protein CAMSAP2 controls axon specification and dendrite development. Neuron. 82(5):1058-73. 2014.
- Hu WF, Pomp O, Ben-Omran T. Katanin p80 regulates human cortical development by limiting centriole and cilia number. Neuron. 84(6):1240-57. 2014.
- van Beuningen SF, Will L, Harterink M. TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays. Neuron. 88(6):1208-26. 2015.
- Yau KW, Schätzle P, Tortosa E. Dendrites In Vitro and In Vivo Contain Microtubules of Opposite Polarity and Axon Formation Correlates with Uniform Plus-End-Out Microtubule Orientation. The Journal of neuroscience : the official journal of the Society for Neuroscience. 36(4):1071-85. 2016.
- Tanaka N, Meng W, Nagae S, Takeichi M. Nezha/CAMSAP3 and CAMSAP2 cooperate in epithelial-specific organization of noncentrosomal microtubules. Proceedings of the National Academy of Sciences of the United States of America. 109(49):20029-34. 2012.
- Syred HM, Welburn J, Rappsilber J, Ohkura H. Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition. Molecular & cellular proteomics : MCP. 12(11):3135-47. 2013.
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