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Product Name
CaMKII gamma antibody
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Description
CaMKII gamma Rabbit Polyclonal antibody. Positive WB detected in SMMC-7721 cells, human brain tissue. Positive IP detected in mouse heart tissue. Positive IHC detected in human pancreas cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 59 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CaM kinase II subunit gamma antibody; CAMK antibody; CAMK II antibody; CaMK II subunit gamma antibody; CAMK2G antibody; CAMKG antibody; FLJ16043 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CaMKII gamma recombinant protein (Accession Number: NM_172169). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:108920(CAMK2G antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using CAMK2G antibody Catalog No:108920 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
SMMC-7721 cells were subjected to SDS PAGE followed by western blot with Catalog No:108920(CAMK2G Antibody) at dilution of 1:2000
IP Result of anti-CAMK2G (IP:Catalog No:108920, 4ug; Detection:Catalog No:108920 1:1000) with mouse heart tissue lysate 3200ug.
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Background
CaMKII gamma, also named as CAMKG, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G has been shown to be expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some study can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes.(PMID: 12032636 ) This antibody can recognize all the 9 isoforms of CAMK2G. So the band in WB is from 50-65kd. This antibody can bind all the CAMK2s for the close sequences.
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References
- Chang HY, Minahan K, Merriman JA, Jones KT. Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse. Development (Cambridge, England). 136(24):4077-81. 2009.
- Chen H, Xu L, Yin L. iTRAQ-based proteomic analysis of dioscin on human HCT-116 colon cancer cells. Proteomics. 14(1):51-73. 2014.
- Li C, Zhou X, Chen J. PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1. Oncotarget. 6(29):27794-804. 2015.
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