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Product Name
Calponin antibody
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Description
Calponin Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human bladder tissue, human breast cancer tissue, human hysteromyoma tissue, human ovary tumor tissue, human skin tissue. Positive IP detected in mouse lung tissue. Positive WB detected in mouse heart tissue, mouse lung tissue. Observed molecular weight by Western-blot: 35kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Basic calponin antibody; Calponin antibody; Calponin 1 antibody; Calponin H1 antibody; smooth muscle antibody; CNN1 antibody; Sm Calp antibody; SMCC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Calponin recombinant protein (Accession Number: NM_001299). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human heart using Catalog No:108905(CNN1 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:108905(CNN1 antibody) at dilution of 1:50 (under 10x lens)
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108905(CNN1 antibody) at dilution of 1:1000
IP Result of anti-Calponin (IP:Catalog No:108905, 4ug; Detection:Catalog No:108905 1:1000) with mouse lung tissue lysate 2800ug.
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Background
Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.
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References
- Liu M, Yang J, Li M. Tanshinone IIA attenuates interleukin-17A-induced systemic sclerosis patient-derived dermal vascular smooth muscle cell activation via inhibition of the extracellular signal-regulated kinase signaling pathway. Clinics (São Paulo, Brazil). 70(4):250-6. 2015.
- Han Y, Slivano OJ, Christie CK, Cheng AW, Miano JM. CRISPR-Cas9 genome editing of a single regulatory element nearly abolishes target gene expression in mice--brief report. Arteriosclerosis, thrombosis, and vascular biology. 35(2):312-5. 2015.
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