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Product Name
CALCOCO2 antibody
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Description
CALCOCO2 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue, human skeletal muscle tissue. Positive WB detected in HeLa cells, human placenta tissue, Jurkat cells, mouse skeletal muscle tissue, rat skeletal muscle tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CALCOCO2 antibody; NDP52 antibody; Nuclear domain 10 protein 52 antibody; Nuclear dot protein 52 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CALC℃O2 recombinant protein (Accession Number: NM_005831). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108818(CALCocO2 antibody) at dilution of 1:1000
IP Result of anti-CALCocO2 (IP:Catalog No:108818, 3ug; Detection:Catalog No:108818 1:1000) with HeLa cells lysate 2150ug.
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:108818(CALCocO2 antibody) at dilution of 1:100 (under 10x lens)
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:108818(CALCocO2 Antibody) at dilution of 1:1000
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Background
CALCocO2, also named as NDP52, is an autophagy receptor with MW 52 kDa. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat CALCocO2 has some isoforms with MW 28-35 kDa.
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References
- Selleck EM, Orchard RC, Lassen KG. A Noncanonical Autophagy Pathway Restricts Toxoplasma gondii Growth in a Strain-Specific Manner in IFN-γ-Activated Human Cells. mBio. 6(5):e01157-15. 2015.
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