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Cadherin-13 antibody

Cat.#: 108772

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Product Information

  • Product Name
    Cadherin-13 antibody
  • Documents
  • Description
    Cadherin-13 Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue, human heart tissue, L02 cells. Positive IP detected in mouse heart tissue. Positive FC detected in Jurkat cells. Positive IHC detected in human endometrial cancer tissue, human heart tissue. Observed molecular weight by Western-blot: 105 kDa
  • Tested applications
    ELISA, WB, IHC, FC, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    Cadherin 13 antibody; Cadherin-13 antibody; CDH13 antibody; CDHH antibody; H cadherin antibody; H-cadherin antibody; Heart cadherin antibody; P105 antibody; T cad antibody; T cadherin antibody; T-cadherin antibody; Truncated cadherin antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Cadherin-13 recombinant protein (Accession Number: NM_001257). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108772(CDH13 antibody) at dilution of 1:1000

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108772(CDH13 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:108772(CDH13 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:108772(CDH13 antibody) at dilution of 1:100 (under 10x lens)

    1X10^6 Jurkat cells were stained with 0.5ug CDH13 antibody (Catalog No:108772, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 Jurkat cells were stained with 0.5ug CDH13 antibody (Catalog No:108772, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    IP Result of anti-CDH13 (IP:Catalog No:108772, 4ug; Detection:Catalog No:108772 1:700) with mouse heart tissue lysate 4000ug.

    IP Result of anti-CDH13 (IP:Catalog No:108772, 4ug; Detection:Catalog No:108772 1:700) with mouse heart tissue lysate 4000ug.

  • Background
    Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Cadherin-13 (CDH13, also known as H-cadherin or T-cadherin) is an atypical member of cadherin family, lacking transmembrane and cytosolic domains and anchored to the exterior surface of the plasma membrane via a GPI anchor. CDH13 is thought to affect cellular behavior largely through its signaling properties. It is highly expressed in heart, and in the CNS, CDH13 is expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. It is often down-regulated in cancerous cells. The CDH13 cDNA encodes a 713-amino acid protein, which has a calculated molecular weight of 78 kDa. Studies have detected variable bands on Western blots, including 105 kDa (mature form), 130 kDa (precursor), and 45 kDa, which may be a post-translational processing variant of CDH13 (PMID: 19399994).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"