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Product Name
CA13 antibody
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Description
CA13 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, mouse skeletal muscle tissue. Positive IF detected in NIH/3T3 cells. Positive IHC detected in human placenta tissue, human heart tissue, human kidney tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CA XIII antibody; CA13 antibody; Carbonate dehydratase XIII antibody; Carbonic anhydrase 13 antibody; carbonic anhydrase XIII antibody; CAXIII antibody; FLJ37995 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CA13 recombinant protein (Accession Number: NM_198584). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:108745(CA13 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human placenta using Catalog No:108745(CA13 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:108745(CA13 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using Catalog No:108745(CA13 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Carbonic anhydrases (CAs) are zinc-containing metalloenzymes that catalyze reversible hydration of carbon dioxide. The mammalian α-CA gene family has been reported to include at least eleven enzymatically active isoforms with different structural and catalytic properties. Four of the active CA isozymes are cytosolic (CA I, II, III, and VII), four are membrane-associated(CA IV, IX, XII, and XIV), two are mitochondrial (CA VA and VB), and one is a secretory form (CA VI). CA13 is a cytosolic proteinmay that may play a role in embryogenesis and perturbation of its function by genetic modification could potentially lead to developmental abnormalities(PMID:14600151).
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