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Product Name
C14orf166 antibody
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Description
C14orf166 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human liver tissue, human skeletal muscle tissue. Positive IF detected in HepG2 cells. Positive IP detected in Jurkat cells. Positive WB detected in mouse thymus tissue, human heart tissue, Jurkat cells, mouse heart tissue, mouse lung tissue, mouse pancreas tissue. Observed molecular weight by Western-blot: 28kd
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C14orf166 antibody; CGI 99 antibody; CGI99 antibody; CLE antibody; CLE7 antibody; LCRP369 antibody; RLLM1 antibody; UPF0568 protein C14orf166 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of C14orf166 recombinant protein (Accession Number: NM_016039). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:108661(CLE; C14orf166 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human heart using Catalog No:108661(CLE; C14orf166 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using C14orf166 antibody Catalog No:108661 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CLE; C14orf166 (IP:Catalog No:108661, 3ug; Detection:Catalog No:108661 1:700) with Jurkat cells lysate 1080ug.
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Background
Human CLE/C14orf166 protein is a positive modulator of cellular RNA polymerase. CLE is also shown to interact with the influenza virus polymerase complex and colocalizes with viral ribonucleoproteins, and is required for viral replication. Similarly, CLE is identified as a novel host interacting partners of the mature hepatitis C virus core protein. CLE levels were significantly higher in the serum of patients with PaCa compared with the control group was strongly expressed in tumor cells, suggesting that C14orf166 may be a potential biomarker of pancreatic adenocarcinoma.
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References
- Zhang W, Ou J, Lei F. C14ORF166 overexpression is associated with pelvic lymph node metastasis and poor prognosis in uterine cervical cancer. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2015.
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