-
Product Name
BubR1 antibody
- Documents
-
Description
BubR1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in SKOV-3 cells. Positive WB detected in mouse testis tissue, HeLa cells, human testis tissue, K-562 cells. Observed molecular weight by Western-blot: 120-130 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
Bub1A antibody; BUB1B antibody; BUB1beta antibody; BubR1 antibody; hBUBR1 antibody; MAD3L antibody; Protein SSK1 antibody; SSK1 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of BubR1 recombinant protein (Accession Number: NM_001211). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:10-1:100
IF: 1:20-1:200
-
Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:108553(BUB1B antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108553(BUB1B antibody) at dilution of 1:10 (under 10x lens)
Immunofluorescent analysis of SKOV-3 cells using Catalog No:108553(BUB1B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).
-
References
- Yuan YF, Zhai R, Liu XM, Khan HA, Zhen YH, Huo LJ. SUMO-1 plays crucial roles for spindle organization, chromosome congression, and chromosome segregation during mouse oocyte meiotic maturation. Molecular reproduction and development. 81(8):712-24. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"