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Product Name
BTF3 Polyclonal Antibody
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Description
Polyclonal antibody to BTF3
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
BTF3 antibody; BETA-NAC antibody; BTF3a antibody; BTF3b antibody; NACB antibody; transcription factor BTF3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human BTF3 (NP_001198.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
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Validations
Western blot - BTF3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BTF3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10S.
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Background
When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"