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Product Name
BTD antibody
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Description
BTD Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human placenta tissue. Positive WB detected in HeLa cells, human brain tissue. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Biotinase antibody; biotinidase antibody; BTD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BTD recombinant protein (Accession Number: NM_000060). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108539(BTD antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human placenta using Catalog No:108539(BTD antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:108539(BTD antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:108539(BTD Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
BTD represents biotinidase
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"