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Product Name
BRMS1 Polyclonal Antibody
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Description
Polyclonal antibody to BRMS1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
BRMS1 antibody; breast cancer metastasis-suppressor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 90-210 of human BRMS1 (NP_056214.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - BRMS1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BRMS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - BRMS1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using BRMS1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BRMS1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using BRMS1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BRMS1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using BRMS1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BRMS1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using BRMS1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BRMS1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using BRMS1 antibody at dilution of 1:100 (40x lens).
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Background
Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"