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Product Name
BNIP3 Polyclonal Antibody
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Description
Polyclonal antibody to BNIP3
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
BNIP3 antibody; NIP3 antibody; BCL2 interacting protein 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human BNIP3 (NP_004043.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - BNIP3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BNIP3 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .
Immunohistochemistry - BNIP3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using BNIP3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BNIP3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BNIP3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using BNIP3 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - BNIP3 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using BNIP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"