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Product Name
BMP15 Polyclonal Antibody
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Description
Polyclonal antibody to BMP15
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
BMP15 antibody; GDF9B antibody; ODG2 antibody; POF4 antibody; bone morphogenetic protein 15 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 143-392 of human BMP15 (NP_005439.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - BMP15 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BMP15 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 30s.
Immunohistochemistry - BMP15 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using BMP15 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BMP15 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using BMP15 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BMP15 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using BMP15 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BMP15 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using BMP15 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - BMP15 Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using BMP15 antibody . Blue: DAPI for nuclear staining.
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Background
May be involved in follicular development. Oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"