-
Product Name
Human BLMH/Bleomycin Hydrolase (His Tag) recombinant protein
- Documents
-
Description
The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.
-
Protein name
Bleomycin hydrolase
-
Protein short names
AI035728; RP23-100P23.3; MGC36899; BMH; MGC39026; BLEOMYCIN HYDROLASE; BH; MGC37104; BLMH
-
Uniprot ID
Q13867
-
Gene Name
BLMH
-
Source/Expression Host
E. coli
-
Expression Plasmid/cDNA
A DNA sequence encoding the human BLMH (NP_000377.1) (Ser 2-Glu 455) was expressed, with a polyhistide tag at the N-terminus.
-
Protein Species
Human
-
Molecular weight
The recombinant human BLMH consisting of 461 amino acids and has a calculated molecular mass of 53.4 kDa as estimated in SDS-PAGE under reducing conditions.
-
Purity
> 88 % as determined by SDS-PAGE
-
Activity
Measured by its ability to hydrolyze Met-AMC. The specific activity is >500 pmoles/min/μg.
-
Validations
Human BLMH / BLM hydrolase Protein (His Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"