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Product Name
Mouse BLMH/Bleomycin Hydrolase (His Tag) recombinant protein
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Description
The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.
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Protein name
Bleomycin hydrolase
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Protein short names
MGC39026; BMH; BLEOMYCIN HYDROLASE; RP23-100P23.3; MGC36899; AI035728; BLMH; MGC37104; BH
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Uniprot ID
Q8R016
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Gene Name
Blmh
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse BLMH (NP_848760.1) (Asn 2-Glu 455) was expressed, with a polyhistide tag at the N-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse BLMH consisting of 461 amino acids and has a calculated molecular mass of 53.3 kDa. rmBLMH migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its ability to hydrolyze Met-AMC. The specific activity is >500 pmoles/min/μg.
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Validations
Mouse BLMH / Bleomycin Hydrolase Protein (His Tag) SDS-PAGE
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