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BIN3 antibody

Cat.#: 108503

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Product Information

  • Product Name
    BIN3 antibody
  • Documents
  • Description
    BIN3 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells, HeLa cells. Observed molecular weight by Western-blot: 30 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    BIN3 antibody; bridging integrator 3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of BIN3 recombinant protein (Accession Number: NM_018688). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:117148(BIN3 antibody) at dilution of 1:300

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:117148(BIN3 antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:117148(BIN3 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:117148(BIN3 antibody) at dilution of 1:50 (under 10x lens)

    IP Result of anti-BIN3 (IP:Catalog No:117148, 4ug; Detection:Catalog No:117148 1:300) with K-562 cells lysate 3200ug.

    IP Result of anti-BIN3 (IP:Catalog No:117148, 4ug; Detection:Catalog No:117148 1:300) with K-562 cells lysate 3200ug.

  • Background
    Bridging integrator 3 (BIN3) is a conserved BAR (Bin/amphiphysin/ Rvs) domain adaptor protein (PMID: 11274158). BAR domain proteins, sharing an N-terminal BAR domain, have been implicated in diverse cellular processes, including endocytosis, vesicle fusion, cell polarity, actin organization, signal transduction, transcription repression, and tumor suppression (PMID: 16524918). BIN3 is involved in cytokinesis and septation where it has a role in the localization of F-actin (PMID: 11274158).
  • References
    • Hoffmann A, Huang Y, Suetsugu-Maki R. Implication of the miR-184 and miR-204 competitive RNA network in control of mouse secondary cataract. Molecular medicine (Cambridge, Mass.). 18:528-38. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"