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Product Name
BHMT antibody
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Description
BHMT Rabbit Polyclonal antibody. Positive WB detected in human kidney tissue, L02 cells. Positive IP detected in mouse kidney tissue. Positive IHC detected in human spleen tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue. Observed molecular weight by Western-blot: 50kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
BHMT antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BHMT recombinant protein (Accession Number: NM_001713). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:117140(BHMT antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human spleen using Catalog No:117140(BHMT antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:117140(BHMT antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-BHMT (IP:Catalog No:117140, 4ug; Detection:Catalog No:117140 1:1000) with mouse kidney tissue lysate 4400ug.
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Background
BHMT represents betaine-homocysteine methyltransferase
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References
- Wang Y, Kou Y, Wang X, Cederbaum A, Wang R. Multifactorial comparative proteomic study of cytochrome P450 2E1 function in chronic alcohol administration. PloS one. 9(3):e92504. 2014.
- Li X, Sun Q, Li X. Dietary betaine supplementation to gestational sows enhances hippocampal IGF2 expression in newborn piglets with modified DNA methylation of the differentially methylated regions. European journal of nutrition. 54(7):1201-10. 2015.
- Hu Y, Sun Q, Li X. In Ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks. PloS one. 10(4):e0122643. 2015.
- Jia Y, Song H, Gao G, Cai D, Yang X, Zhao R. Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets. Journal of agricultural and food chemistry. 63(46):10152-60. 2015.
- Jia L, Li J, He B. Abnormally activated one-carbon metabolic pathway is associated with mtDNA hypermethylation and mitochondrial malfunction in the oocytes of polycystic gilt ovaries. Scientific reports. 6:19436. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"