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Product Name
beta Tubulin antibody
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Description
beta Tubulin Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in multi-cells/tissue, A431 cells, COLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, Jurkat cells, mouse brain tissue, Neuro-2a cells, NIH/3T3 cells, rat brain tissue, SH-SY5Y cells. Positive IHC detected in mouse brain tissue, human brain tissue, rat brain tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
beta 4 antibody; beta tubulin antibody; MC1R antibody; TUBB antibody; tubulin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of beta Tubulin recombinant protein (Accession Number: NM_006086). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
WB results on multiple cells/tissues with anti-Tubulin-beta (Catalog No:117306) at dilution 1:1,000.
Western blot of Hela cell with anti-Tubulin-Beta (Catalog No:117306) at various dilutions.
IP Result of anti-Tubulin-beta (IP:Catalog No:117306, 3ug; Detection:Catalog No:117306 1:1000) with mouse brain tissue lysate 3600ug.
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:117306(Tubulin-beta Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:117306(Tubulin-beta Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:117306(Tubulin-beta Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
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References
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- Li Y, Wang S, Wang Z. Cationic poly(amidoamine) dendrimers induced cyto-protective autophagy in hepatocellular carcinoma cells. Nanotechnology. 25(36):365101. 2014.
- Peng L, Yang J, Ning C. Rhein inhibits integrin-linked kinase expression and regulates matrix metalloproteinase-9/tissue inhibitor of metalloproteinase-1 ratio in high glucose-induced epithelial-mesenchymal transition of renal tubular cell. Biological & pharmaceutical bulletin. 35(10):1676-85. 2012.
- He Q, Zhang C, Wang L. Inflammatory signaling regulates hematopoietic stem and progenitor cell emergence in vertebrates. Blood. 125(7):1098-106. 2015.
- Qin S, Xu C, Li S. Indomethacin induces apoptosis in the EC109 esophageal cancer cell line by releasing second mitochondria-derived activator of caspase and activating caspase-3. Molecular medicine reports. 11(6):4694-700. 2015.
- Zhai Y, Wu B, Li J, Yao XY, Zhu P, Chen ZN. CD147 promotes IKK/IκB/NF-κB pathway to resist TNF-induced apoptosis in rheumatoid arthritis synovial fibroblasts. Journal of molecular medicine (Berlin, Germany). 94(1):71-82. 2016.
- Zhang Y, Wang Y, Wei Y. MiR-129-3p promotes docetaxel resistance of breast cancer cells via CP110 inhibition. Scientific reports. 5:15424. 2015.
- Lan X, Li L, Hu J, Zhang Q, Dang Y, Huang Y. A Quantitative Method for Microtubule Analysis in Fluorescence Images. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada. 21(6):1582-1590. 2015.
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