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beta Actin antibody

Cat.#: 107087

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Product Information

  • Product Name
    beta Actin antibody
  • Documents
  • Description
    beta Actin Mouse Monoclonal antibody. Positive WB detected in multi-cells/tissue, A549 cells, arabidopsis whole plant tissue, HEK-293 cells, HeLa cells, MCF7 cells, whole Nematode tissue, whole yeast cells. Positive IHC detected in human kidney tissue, human brain tissue, human colon tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 42 kDa
  • Tested applications
    ELISA, IHC, WB, IF, FC
  • Species reactivity
    Human, Mouse, Rat, Zebrafish, Pig, yeast; other species not tested.
  • Alternative names
    ACTB antibody; Actin antibody; actin antibody; beta antibody; Actin antibody; cytoplasmic 1 antibody; B actin antibody; Beta actin antibody; F actin antibody; β actin antibody
  • Isotype
    Mouse IgM
  • Preparation
    This antibody was obtained by immunization of beta Actin recombinant protein (Accession Number: NM_001101). Purification method: Caprylic acid/ammonium sulfate precipitation.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:2000-1:20000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    Western blot analysis of Beta-actin in various tissues and cell lines using Proteintech antibody Catalog No:117304 at a dilution of 1:5000. Extra bands were detected in some species with unknown reason.

    Western blot analysis of Beta-actin in various tissues and cell lines using Proteintech antibody Catalog No:117304 at a dilution of 1:5000. Extra bands were detected in some species with unknown reason.

    Immunofluorescent analysis of HepG2 cells using Catalog No:117304 (beta actin Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

    Immunofluorescent analysis of HepG2 cells using Catalog No:117304 (beta actin Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

    Western blot of Hela cell with anti-Actin-Beta (Catalog No:117304) at various dilutions.

    Western blot of Hela cell with anti-Actin-Beta (Catalog No:117304) at various dilutions.

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:117304(ACTB antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:117304(ACTB antibody) at dilution of 1:100 (under 40x lens)

    1X10^6 HepG2 cells were stained with 0.2ug beta actin antibody (Catalog No:117304, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with 0.2ug beta actin antibody (Catalog No:117304, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

  • Background
    Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
  • References
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