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Product Name
BEND3 antibody
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Description
BEND3 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue. Observed molecular weight by Western-blot: 85; 94 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
BEN domain containing 3 antibody; BEND3 antibody; KIAA1553 antibody; RP11 59I9.2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BEND3 recombinant protein (Accession Number: XM_017011138). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:50-1:500
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:117116(BEND3 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:117116(BEND3 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:117116(BEND3 Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:117116(BEND3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
BEND3 has 2 isoforms with MW 85 kDa (743 aa) and 94 kDa (828 aa). Catalog #23101-1-AP specially recognizes 85 and 94 kDa isoforms.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"