-
Product Name
BDNF antibody
- Documents
-
Description
BDNF Mouse Monoclonal antibody. Positive IF detected in PC-12 cells. Positive WB detected in U-251 cells, fetal human brain tissue. Observed molecular weight by Western-blot: 32 kDa
-
Tested applications
ELISA, WB, IF
-
Species reactivity
Human, Rat; other species not tested.
-
Alternative names
Abrineurin antibody; BDNF antibody
-
Isotype
Mouse IgG2a
-
Preparation
This antibody was obtained by immunization of BDNF recombinant protein (Accession Number: NM_001143805). Purification method: Protein A purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:50-1:500
-
Validations
U-251 cells were subjected to SDS PAGE followed by western blot with Catalog No:107084(BDNF Antibody) at dilution of 1:1000
Immunofluorescent analysis of (10% Formaldehyde) fixed PC-12 cells using Catalog No:107084(BDNF Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
-
Background
BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. It play a role in the regulation of stress response and in the biology of mood disorders.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"