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Product Name
BCL9 antibody
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Description
BCL9 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, Transfected HEK-293 cells. Positive IHC detected in human liver cancer tissue, human colon cancer tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 149 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
B cell CLL/lymphoma 9 antibody; B cell CLL/lymphoma 9 protein antibody; B cell lymphoma 9 protein antibody; BCL9 antibody; LGS antibody; Protein legless homolog antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BCL9 recombinant protein (Accession Number: NM_004326). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:117104(BCL9 Antibody) at dilution of 1:600
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:117104(BCL9 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:117104(BCL9 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:117104(BCL9 Antibody) at dilution of 1:200 (under 40x lens).
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:117104(BCL9 Antibody) at dilution of 1:1000
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Background
BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.
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