-
Product Name
BCHE Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to BCHE
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
BCHE antibody; CHE1 antibody; CHE2 antibody; E1 antibody; cholinesterase antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-270 of human BCHE (NP_000046.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - BCHE Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BCHE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - BCHE Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using BCHE antibody at dilution of 1:100 (40x lens).
Immunofluorescence - BCHE Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using BCHE antibody . Blue: DAPI for nuclear staining.
-
Background
Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"