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Product Name
Anti-CD31/PECAM-1 antibody
- Documents
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Description
Rabbit polyclonal to CD31/PECAM-1
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse CD31 / PECAM-1
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Alternative names
CD31 antibody; PECAM1 antibody; PECAM-1 antibody; Cd31 antibody; Pecam antibody; C85791 antibody; PECAM-1 antibody; CD31 antibody; PECA1 antibody; GPIIA' antibody; PECAM-1 antibody; endoCAM antibody; CD31/EndoCAM antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse CD31 / PECAM-1 (rh CD31 / PECAM-1; NP_033868.3; Met1-Lys590). CD31 / PECAM-1 specific IgG was purified by Mouse CD31 / PECAM-1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CD31 / PECAM-1. The detection limit for Mouse CD31 / PECAM-1 is 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
CD31 / PECAM-1 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of mouse PECAM1 in mouse brain with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
CD31 / PECAM-1 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of mouse PECAM1 in mouse heart with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.
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References
- Deaglio S, et al. (2000) CD38/CD31, a receptor/ligand system ruling adhesion and signaling in human leukocytes. Chem Immunol. 75: 99-120.
- Kohler S, et al. (2009) Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood. 113(4): 769-74.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"