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BCCIP antibody

Cat.#: 108384

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Product Information

  • Product Name
    BCCIP antibody
  • Documents
  • Description
    BCCIP Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A549 cells. Positive IP detected in HeLa cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 50-58 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    BCCIP antibody; Protein TOK 1 antibody; TOK 1 antibody; TOK1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of BCCIP recombinant protein (Accession Number: NM_078468). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IF: 1:50-1:500

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108384(BCCIP antibody) at dilution of 1:800

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108384(BCCIP antibody) at dilution of 1:800

    IP Result of anti-BCCIP (IP:Catalog No:108384, 4ug; Detection:Catalog No:108384 1:800) with HeLa cells lysate 3200ug.

    IP Result of anti-BCCIP (IP:Catalog No:108384, 4ug; Detection:Catalog No:108384 1:800) with HeLa cells lysate 3200ug.

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:108384(BCCIP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:108384(BCCIP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    BCCIP (or TOK1) is isolated as a BRCA2- and CDKN1A (p21)-interacting protein and is implicated in homologous recombination (HR) pathway and inhibition of DNA replication stress. BCCIP is an evolutionarily conserved nuclear protein with multiple interacting domains. BCCIP deficiency in mice impaired embryonic and postnatal neural development, causing severe ataxia, cerebral and cerebellar defects, and microcephaly, which are associated with spontaneous DNA damage and subsequent cell death in the proliferative cell populations of the neural system during embryogenesis. BCCIP is essential for maintaining the transactivation activity of wild type p53 suggesting a potential role of BCCIP in cancer etiology.
  • References
    • Liu X, Cao L, Ni J. Differential BCCIP gene expression in primary human ovarian cancer, renal cell carcinoma and colorectal cancer tissues. International journal of oncology. 43(6):1925-34. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"