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Product Name
BAZ1B Polyclonal Antibody
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Description
Polyclonal antibody to BAZ1B
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
BAZ1B antibody; WBSCR10 antibody; WBSCR9 antibody; WSTF antibody; tyrosine-protein kinase BAZ1B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BAZ1B (NP_115784.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - BAZ1B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BAZ1B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Western blot - BAZ1B Polyclonal Antibody
Western blot analysis of extracts from normal (control) and BAZ1B knockout HeLa cells, using BAZ1B antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunohistochemistry - BAZ1B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using BAZ1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BAZ1B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using BAZ1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BAZ1B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BAZ1B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse stomach using BAZ1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BAZ1B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using BAZ1B antibody at dilution of 1:100 (40x lens).
Immunofluorescence - BAZ1B Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using BAZ1B antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"