-
Product Name
BAAT antibody
- Documents
-
Description
BAAT Rabbit Polyclonal antibody. Positive IHC detected in human hepat℃irrhosis tissue. Positive IF detected in HepG2 cells. Positive WB detected in human liver tissue. Observed molecular weight by Western-blot: 50kd
-
Tested applications
ELISA, WB, IF, IHC
-
Species reactivity
Human; other species not tested.
-
Alternative names
BAAT antibody; BACAT antibody; BAT antibody; FLJ20300 antibody; Glycine N choloyltransferase antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of BAAT recombinant protein (Accession Number: NM_001374715). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:108400(BAAT antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using Catalog No:108400(BAAT Antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using Catalog No:108400(BAAT Antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:108400(BAAT Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
BAAT is bile acid-CoA:amino acid N-acyltransferase which involved in in bile acid metabolism. BAAT acts as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. BAAT protein showes 46-50 kDa in WB detection.
-
References
- Chatterjee S, Bijsmans IT, van Mil SW, Augustijns P, Annaert P. Toxicity and intracellular accumulation of bile acids in sandwich-cultured rat hepatocytes: role of glycine conjugates. Toxicology in vitro : an international journal published in association with BIBRA. 28(2):218-30. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"