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Product Name
B4GALT6 antibody
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Description
B4GALT6 Rabbit Polyclonal antibody. Positive IHC detected in human colon tissue, human brain tissue. Positive IF detected in Hela cells. Positive WB detected in mouse heart tissue, HEK-293 cells, human brain tissue, human heart tissue, human placenta tissue, mouse brain tissue, mouse kidney tissue. Observed molecular weight by Western-blot: 45-60kd
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B4Gal T6 antibody; B4GALT6 antibody; Beta 1 antibody; 4 GalTase 6 antibody; beta4Gal T6 antibody; LacCer synthase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of B4GALT6 recombinant protein (Accession Number: NM_004775). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human colon using Catalog No:108396(B4GALT6 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon using Catalog No:108396(B4GALT6 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using B4GALT6 antibody Catalog No:108396 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108396(B4GALT6 Antibody) at dilution of 1:600
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Background
B4GALT5 is a member of the beta 4-galactosyltransferase family that catalyze LacCer synthesis. LacCer is a common precursor for the synthesis of all glycosphingolipids derived from GlcCer. High expression of B4GALT6 and high LacCer concentrations were found in multiple sclerosis (MS) lesions, and B4GALT6 in astrocytes has been identified as a driver of chronic CNS inflammation. (25216636)
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References
- Mayo L, Trauger SA, Blain M. Regulation of astrocyte activation by glycolipids drives chronic CNS inflammation. Nature medicine. 20(10):1147-56. 2014.
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