AXL Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive WB detected in HepG2 cells, HeLa cells, HT-1080 cells, human liver tissue. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 140 kDa
ELISA, WB, IP, FC
Human; other species not tested.
AXL antibody; AXL oncogene antibody; AXL receptor tyrosine kinase antibody; JTK11 antibody; UFO antibody
This antibody was obtained by immunization of AXL recombinant protein (Accession Number: BC032229). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:117080(AXL antibody) at dilution of 1:1500
IP Result of anti-AXL (IP:Catalog No:117080, 5ug; Detection:Catalog No:117080 1:1000) with HepG2 cells lysate 9000ug.
1X10^6 HeLa cells were stained with 0.2ug AXL antibody (Catalog No:117080, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. This antibody detects a single band at 140 kDa, which is considered to represent the auto-phosphorylated form of endogenous Axl (PMID: 12730092).
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