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Product Name
ATP6V0D1 antibody
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Description
ATP6V0D1 Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human kidney tissue. Positive IP detected in mouse testis tissue. Positive WB detected in mouse testis tissue, HeLa cells, human placenta tissue, mouse kidney tissue. Observed molecular weight by Western-blot: 41 kDa
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Tested applications
ELISA, WB, IHC, FC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
32 kDa accessory protein antibody; ATP6D antibody; ATP6DV antibody; ATP6V0D1 antibody; P39 antibody; V ATPase AC39 subunit antibody; V ATPase subunit d 1 antibody; VATX antibody; VMA6 antibody; VPATPD antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATP6V0D1 recombinant protein (Accession Number: NM_004691). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:108303(ATP6V0D1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human kidney using Catalog No:108303(ATP6V0D1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:108303(ATP6V0D1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ATP6V0D1 (IP:Catalog No:108303, 4ug; Detection:Catalog No:108303 1:500) with mouse testis tissue lysate 4800ug.
Immunofluorescent analysis of HeLa cells using Catalog No:108303(ATP6V0D1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug ATP6V0D1 antibody (Catalog No:108303, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.
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References
- Otomo T, Higaki K, Nanba E, Ozono K, Sakai N. Lysosomal storage causes cellular dysfunction in mucolipidosis II skin fibroblasts. The Journal of biological chemistry. 286(40):35283-90. 2011.
- Zeng HL, Rao X, Zhang LK. Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome. Journal of proteomics. 109:125-42. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"