Product Name

ATP5A1 antibody

Documents

Description

ATP5A1 Rabbit Polyclonal antibody. Positive WB detected in multi-cells/tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF7 cells, mouse heart tissue, mouse liver tissue, PC-3 cells, rat heart tissue, Y79 cells. Positive IHC detected in human liver tissue, human kidney tissue. Positive IF detected in HepG2 cells, Hela cells. Observed molecular weight by Western-blot: 50-55kd

Tested applications

ELISA, IHC, WB, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

ATP5A antibody; ATP5A1 antibody; ATP5AL2 antibody; ATPM antibody; hATP1 antibody; MOM2 antibody; OMR antibody; ORM antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of ATP5A1 recombinant protein (Accession Number: NM_004046). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

WB results of Catalog No:108350 (anti-ATP5A1 antibody).

Immunofluorescent analysis of HepG2 cells, using ATP5A1 antibody Catalog No:108350 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunohistochemical of paraffin-embedded human liver using Catalog No:108350(ATP5A1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human liver using Catalog No:108350(ATP5A1 antibody) at dilution of 1:100 (under 40x lens)

Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:108350(ATP5A1 Antibody) at dilution of 1:50

Background

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a,b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues.

References

Preuss B, Berg C, Dengjel J, Stevanovic S, Klein R. Relevance of the inner mitochondrial membrane enzyme F1F0-ATPase as an autoantigen in autoimmune liver disorders. Liver international : official journal of the International Association for the Study of the Liver. 32(2):249-57. 2012.
Plun-Favreau H, Burchell VS, Holmström KM. HtrA2 deficiency causes mitochondrial uncoupling through the F₁F₀-ATP synthase and consequent ATP depletion. Cell death & disease. 3:e335. 2012.
Shen C, Kuang Y, Liu J. Prss37 is required for male fertility in the mouse. Biology of reproduction. 88(5):123. 2013.
Meierhofer D, Weidner C, Hartmann L. Protein sets define disease states and predict in vivo effects of drug treatment. Molecular & cellular proteomics : MCP. 12(7):1965-79. 2013.
Fowler SL, Akins M, Zhou H, Figeys D, Bennett SA. The liver connexin32 interactome is a novel plasma membrane-mitochondrial signaling nexus. Journal of proteome research. 12(6):2597-610. 2013.
Kantardzhieva A, Peppi M, Lane WS, Sewell WF. Protein composition of immunoprecipitated synaptic ribbons. Journal of proteome research. 11(2):1163-74. 2012.
Kioka H, Kato H, Fujikawa M. Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation. Proceedings of the National Academy of Sciences of the United States of America. 111(1):273-8. 2014.
Wang Y, Zhang Y, Zhu Y, Zhang P. Lipolytic inhibitor G0/G1 switch gene 2 inhibits reactive oxygen species production and apoptosis in endothelial cells. American journal of physiology. Cell physiology. 308(6):C496-504. 2015.

ATP5A1 antibody

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